1x te buffer (Thermo Fisher)

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Thermo Fisher 1x te buffer
1x Te Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+te+buffer/TE+Buffer%2C+Solution%2C+Molecular+Biology+Grade%2C+Ultrapure/pm42120873-67-32-35
Average 94 stars, based on 1 article reviews

1x te buffer - by Bioz Stars, 2026-09

94/100 stars

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Lysis:

Article Title: A new genome assembly of the pea cultivar 'Caméor' provides resources for functional genomics and genetics.
Article Snippet: .. After overnight proteinase K digestion in the presence of Lysis Buffer and one hour treatment with RNAse A (Qiagen), plugs were washed three times in 1x Wash Buffer and three times in 1x TE Buffer (ThermoFisher Scientific). .. Then, plugs were melted two minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific) for 45 minutes at 43°C.

Concentration Assay:

Article Title: Systems-level phosphoproteomics reveals conserved and subunit-specific STRIPAK signaling networks in Cryptococcus neoformans
Article Snippet: Genomic DNA for whole-genome sequencing was extracted from YPD-grown cells using the MasterPure Yeast DNA Purification Kit (LGC Biosearch Technologies, MPY80200). .. Precipitated DNA was dissolved in 1x TE buffer (100 mM Tris-HCl, 10 mM EDTA, pH= 8.0) and concentration and purity were estimated using Qubit and Nanodrop, respectively. .. Illumina sequencing was performed at the Duke Sequencing and Genomic Technologies core facility with Novaseq 6000 providing 150 bp paired-end reads.

Centrifugation:

Article Title: Structural analysis of 23S rRNA methylating enzyme Cfr reveals RNA binding determinants for methylation regiospecificity and antibiotic resistance
Article Snippet: Gels were visualized in a BioRad ChemiDoc MP Imaging System, and the single rRNA fragment band was sliced in a Benchtop 2UV Transilluminator at 302 nm. .. Gel pieces were extracted in 1X TE buffer by heat and freeze cycles, and gel was removed with Spin X-centrifuge tubes (Costar 8160). rRNA was precipitated overnight in the presence of 1 μL glycoblue (Invitrogen AM9515), recovered by centrifugation, resuspended in an 18 μL solution of 0.14 M 3-Hydroxypicolinic acid (3-HPA) (Thermo Scientific 241050050) and 500 U of T1 nuclease (Thermo Scientific 01221951), and incubated for 3 hours at 37°C. .. rRNA was precipitated overnight in the presence of 1 μL glycoblue (Invitrogen AM9515), recovered by centrifugation, resuspended in an 18 μL solution of 0.14 M 3-Hydroxypicolinic acid (3-HPA) (Thermo Scientific 241050050) and 500 U of T1 nuclease (Thermo Scientific 01221951), and incubated for 3 hours at 37°C.

Incubation:

other:

Article Title: Structural Analysis of 23S rRNA Methylating Enzyme Cfr Reveals RNA-Binding Determinants for Methylation Regiospecificity and Antibiotic Resistance.
Article Snippet: Total RNA was purified using the RNeasy Midi Kit from Qiagen (Toronto, Ontario, Canada) by the manufacturer’s protocol.

Formulation:

Article Title: Resolving heterogeneity of targeted lipid nanoparticles through solution-based biophysical analyses
Article Snippet: Relative encapsulation efficiency and encapsulated RNA concentration were determined using a Quant-iT RiboGreen assay (Thermo Fisher Scientific). .. Each LNP formulation was diluted 80X in either 1X tris-EDTA (TE) buffer (Thermo Fisher Scientific) or 1X TE buffer containing 0.1% (v/v) Triton X-100 (Sigma Aldrich). ..

Extraction:

Article Title: Investigating contagious transmission of Mannheimia haemolytica in feedlot calves by leveraging whole genome sequences of a unique isolate collection.
Article Snippet: The role of specific strains of Mannheimia haemolytica (Mh) acting as contagious pathogens in the bovine respiratory disease complex remains debated.. This study evaluated the contagious spread of Mh within and between adjacent feedlot pens using whole-genome-sequencing (WGS) to assess microbial genetic relationships.. Deepnasopharyngeal swabs were collected from auction-market calves entering a Saskatchewan feedlot in fall 2020; Mh isolates from 400 steers housed in four contiguous pens (100 steers per pen) were sequenced.

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